Microbacterium phage CarmelMach
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| Detailed Information for Phage CarmelMach | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | CarmelMacch |
| Found By | Emily Borish |
| Year Found | 2019 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.9575 N, 86.47111 W Map |
| Discovery Notes | This sample was taken from Lost River Cave in Bowling Green, Kentucky. It was purified four times and the last purification was a big/small test was carried out to see if the different morphologies seen were different phages. Since the big and small plaques were seen in the same ratio in serial dilutions using the big and small plaques individually, it was concluded that they were all the same phage. After this, the research moved on to making a one plate lysate and then a ten plate lysate. The ten plate lysate has a titre of 6.5 x 10^8 pfu/mL, instead of moving onto DNA isolation, ten more webby plates were made, flooded and titred in hopes that the titre would raise. Instead, most of the plates showed no plaques and a true titre couldn't be calculated. A third ten plate lysate was created. It had a titre of 1.6 x 10^8 pfu/mL. The first ten plate lysate was used to make the electron microscope sample. The phage's DNA was isolated using both the first and third ten plate lysate. The DNA had a concentration of 85.4 ng/uL. A gel electorphorisis was able to be run and only showed cuts with the enzyme HaeIII. |
| Naming Notes | Named after my favorite coffee order, Iced Carmel Macchiato |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | CarmelMach has haloed plaques. In some of the serial dilutions both big and small plaques, with diameters of 2 mm and .5 mm respectively, were seen. They were all formed by the same page population. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 6.5x10^8 |
| Pitt Freezer Box# | 100 |
| Pitt Freezer Box Grid# | C8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |