Mycobacterium phage CasaVerde
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Detailed Information for Phage CasaVerde | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Jenna Stoneroad |
Year Found | 2019 |
Location Found | Elizabethville, PA United States |
Finding Institution | Saint Joseph's University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 40.5527 N, 76.8034 W Map |
Discovery Notes | Soil was collected on September 1st, 2019 at 8:10pm in Elizabethville, PA in the front yard of 129 Green Acres Avenue approximately 15 feet from a driveway and 20 feet from the front of the house overlooking a trailer. The soil condition was dry, dark in color and fine. Soil depth was recorded as 6.35 cm. Ambient temperature at the time of collection was 21°C or 70°F with humidity of 76% and weather conditions of cloudy, high humidity, and 3 mph SE wind. |
Naming Notes | The name for this phage came from the fact that it was isolated at my house, Casa, and I wanted to incorporate my mother into the name of my phage. All throughout high school and college, students and teachers called her Kelly Verde in Spanish class so I chose Verde as a more meaningful way to incorporate her into my discovery. I put the two words together which created my phage name CasaVerde. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | Plaques were approximately 2 mm in size, extremely turbid, and circular plaques once doing three rounds of purification and a full plate titer assay. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 2.7×10^9 pfu/mL |
Pitt Freezer Box# | 101 |
Pitt Freezer Box Grid# | G3 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |