Mycobacterium phage Celestial
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| Detailed Information for Phage Celestial | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ashley Otero and Jamie Arabshahi |
| Year Found | 2017 |
| Location Found | West Lafayette, IN USA |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.422956 N, 86.915835 W Map |
| Discovery Notes | A soil sample was gathered on 9/19/17 at 3:10 pm outside of the Biochemistry Building at Purdue University. It was a couple feet from a tree and sidewalk and about 50 feet from the major road, University Street. The sample was taken from surface level in an urban area. It was moderately moist, causing the dirt and mulch to clump together. However, it was not muddy. The moisture may have been either caused from rain in the early morning, or sprinklers. In addition, the sample was taken right next to a drain and was surrounded by lots of leaves. |
| Naming Notes | Kierra Jammer and Abby Murphy used this phage as well due to the inability to locate our own bacteriphages. We enclosed our electron microscopy picture in Digest Picture File because we were afraid to delete the original Celestial group EM file. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Purdue Genomics Facility |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques are roughly 1mm in diameter and are completely clear circles indicating they are lytic phage. At relatively high concentrations, the morphology appears to change so plaques clump together to form one large plaque amongst small ones. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.6E8 |
| Pitt Freezer Box# | 60 |
| Pitt Freezer Box Grid# | A5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
