Mycobacterium phage Chenzo
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Detailed Information for Phage Chenzo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChristina DiMaggio
Year Found2022
Location FoundMerion Station, PA United States
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates39.9966 N, 75.24308 W Map
Discovery NotesCollected 2-2-2022 9:35am
Merion Station, PA; SJU Maguire campus, Merion Hall Driveway storm drain
Moved large rock under snow and pine needles and dug underneath ~2cm deep
Damp soil with ivy roots and pine needles
-2°C, overcast, 100% humidity, -2°C dew point
Dr. Julia Lee-Soety collected the soil samples
Naming NotesChenzo has been a name in my family for many years and I thought it would be nice to keep the name alive.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesChenzo yields small clear plaques that are around 1 mm in diameter. The plaques emerge after 24 hours in the 37 °C incubator.

MTL titer is 5.33 x 10^9 pfu/ml
HTL titer is 2 x 10^11 pfu/ml

For restricted Digest:
DNA concentration is 153.7 ng/μl
OD 260/280 ratio is 1.87

After measuring six phage particles from EM images:
Average capsid diameter is 85 nm Average tail length is 299 nm.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 146
Pitt Freezer Box Grid# A12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload