Mycobacterium phage Chenzo
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Detailed Information for Phage Chenzo | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Christina DiMaggio |
Year Found | 2022 |
Location Found | Merion Station, PA United States |
Finding Institution | Saint Joseph's University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 39.9966 N, 75.24308 W Map |
Discovery Notes | Collected 2-2-2022 9:35am Merion Station, PA; SJU Maguire campus, Merion Hall Driveway storm drain Moved large rock under snow and pine needles and dug underneath ~2cm deep Damp soil with ivy roots and pine needles -2°C, overcast, 100% humidity, -2°C dew point Dr. Julia Lee-Soety collected the soil samples |
Naming Notes | Chenzo has been a name in my family for many years and I thought it would be nice to keep the name alive. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | Chenzo yields small clear plaques that are around 1 mm in diameter. The plaques emerge after 24 hours in the 37 °C incubator. MTL titer is 5.33 x 10^9 pfu/ml HTL titer is 2 x 10^11 pfu/ml For restricted Digest: DNA concentration is 153.7 ng/μl OD 260/280 ratio is 1.87 After measuring six phage particles from EM images: Average capsid diameter is 85 nm Average tail length is 299 nm. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
Pitt Freezer Box# | 146 |
Pitt Freezer Box Grid# | A12 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |