Mycobacterium phage ChocolateMilk
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| Detailed Information for Phage ChocolateMilk | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Anisha Agrawal |
| Year Found | 2025 |
| Location Found | Richmond, VA United States |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.549423 N, 77.452766 E Map |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Phage release assay: No plaques formed from lysogen supernatant → no spontaneous or induced phage release. Phage sensitivity assay: Bacteria thought to be lysogens were still lysed by the phage → no superinfection immunity. These results indicate the bacteria did not contain an integrated prophage. Conclusion: ChocolateMilk is a lytic phage, not lysogenic. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | plaques were small (~0.2–0.3 mm), circular, and clear/transparent. Edges were sharp and well-defined, with no halo or turbid center. Plaques became well isolated at higher dilutions (especially 10⁻⁴). Clear plaques and rapid lysis are consistent with a lytic life cycle, as infected bacteria are efficiently lysed rather than surviving as lysogens. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
