Mycobacterium phage Clyde2013
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| Detailed Information for Phage Clyde2013 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Aparna Badithe |
| Year Found | 2013 |
| Location Found | La Jolla, CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.876831 N, 117.241043 W Map |
| Discovery Notes | Clyde was not originally found by me. I had to pick a plaque from my lab partner's plate and purify that because my enrichment culture did not yield any phage. My lab partner found her soil sample under a large tree on the UCSD campus in Revelle College. She dug about 1 cm deep into the soil, and the weather that day was quite warm. The temperature was probably around 80 degrees Fahrenheit. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | 454 |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My plaques were medium sized, clear, and haloed towards the end. In the beginning, when I first started purifying my phage, the plaques were quite big. However, as I got to the fourth purification, the plaques became smaller and the halos were very obvious. The most obvious halos were in the third purification. The plaques on my ten plate lysate were larger than I expected them to be. Overall, the plaques changed size quite often and I was not sure why. My one plate lysate plaques had a diameter of about 5mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 1 |
| Pitt Freezer Box Grid# | F7 |