Mycobacterium phage Coolbean
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Detailed Information for Phage Coolbean | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | SmolFry |
Found By | Florisel Rodriguez, Anissia Ojeda |
Year Found | 2019 |
Location Found | Commerce City, CO United States |
Finding Institution | University of Colorado Boulder |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 39.8083 N, 104.9339 W Map |
Discovery Notes | Coolbean was discovered in an cold, wet environment in Commerce City, Colorado. The days following up to obtaining the soil in which Coolbean resided snow fell thus leading to the wet, cold environment of its discovery. The temperature in which the soil was recovered from was about 40 degrees Fahrenheit, with no winds present. |
Naming Notes | The name Coolbean came about due to the environmental factors the phage was founded as well as the size of the plaques. The cool portion was made up as the environment that Coolbean was founded in was cold and wet. As for the Bean in Coolbean, the size of the plaques that were created were small and reminded us of beans as those are small as well. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The morphology of the plaques can be shown in the figures provided, and through this we were able to deduce that the discovered phage undergoes the lytic cycle. We were able to deduce that Coolbean undergoes the lytic cycle due to the presence of clear plaques. Unlike temperate phages which demonstrate a clear/cloudy center and turbid halos, Coolbean only indicates the presence of a clear center. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 2.5x10^11 pfu/mL |
Pitt Freezer Box# | 123 |
Pitt Freezer Box Grid# | A9 |
Available Files | |
Plaque Picture | Download |
EM Picture | Download |