Mycobacterium phage Cutelaba
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| Detailed Information for Phage Cutelaba | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Adel Yasin |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 45.389788 N, 75.637671 W Map |
| Discovery Notes | Phage was isolated from the Kilborn Allotment Garden, in a vegetable garden. Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry. The sequence assembled into one contig of 15,084 bp with 100% query cover, 98.90% identity to Jiminy (B1). The sequence was assembled into a second shorter contig of 6,334 bp with 100.0% query cover, 100.0% identity to MelsMeow (B1). This sequence is incomplete based on the size of other B1 phages. |
| Naming Notes | The name “Cutelaba” was chosen as a playful combination of “cute” and the Latin suffix “-laba,” derived from labium (meaning lip or edge), referencing the plaque’s distinct rounded margins on the agar plate. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The phage consistently produced small, round plaques approximately ~1–2 mm in diameter. Each plaque displayed a subtle bullseye-like appearance, with a slightly darker center and a lighter surrounding zone, but no concentric rings or halo effects. The plaques were slightly turbid, with smooth, well-defined edges, and remained stable in appearance throughout amplification. There was no evidence of spontaneous clear-plaque mutants or mixed morphologies. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
