Streptomyces phage DaRealMyers
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Detailed Information for Phage DaRealMyers
Discovery Information
Isolation HostStreptomyces mirabilis NRRL B-2400
Found ByClifton Cunningham & Oluwasemilore Osinowo
Year Found2024
Location FoundElkridge, MD United States
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates39.197561 N, 76.786496 W Map
Naming NotesOur Phage is really good at killing bacteria, so inspired by one of the killers in the Halloween movies Michael Myers, we wanted to represent that quality.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 4, 2025
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NovaSeq X Plus
Read TypePaired-end reads
Read Length150 bp
Approximate Shotgun Coverage27666
Genome length (bp)39032
Character of genome endsCircularly Permuted
GC Content71.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBC
SubclusterBC1
Cluster Life CycleTemperate
Lysogeny NotesA lysogeny test was performed on DaRealMyers, lasting for sixteen days. Using a spot test on S. mirabilis with serially diluted lysate of DaRealMyers, the life cycle of DaRealMyers could be identified. After sixteen days in the incubator, no mesa was observed on any of the serial dilutions of DaRealMyers . This suggests that DaRealMyers is virulent.
Other Cluster Members
Annotating InstitutionUniversity of Maryland, Baltimore County
Annotation StatusBeing Annotated (Expected completion by 12/4/2025)
Plaque NotesPlaque morphology from a serial dilution of DaRealMyers after 24h incubation time at 30 degrees celsius appeared small, circular, and slightly turbid (not completely clear).
Measurements for DaRealMyers:
Head width: 67.82nm
Head length: 74.9nm
Tail length: 323.64nm
MorphotypeSiphoviridae
Number of Genes53
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 211
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload