Mycobacterium phage DarkChelan
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Detailed Information for Phage DarkChelan
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKatherine Duffy and Lauren Buchberger
Year Found2016
Location FoundChelan, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS CoordinatesUnavailable
Discovery NotesFound under a few inches (2-4in.) of wet rocks in the mountains of Chelan. The fround was cold and hard from recent snow flurries, however there was no snow on the area where the soil was found.
Naming NotesThe name was created using the name of the area in which the sample was found and descriptors of the appearance of the sample before enrichment.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Lysogeny NotesOur phage grew on both a the host Samuel Plaqueson and at 37 degrees C. From this, we know that as our phage was able to infect Samuel Plaqueson, a K-cluster phage, our phage does not belng to this cluster. Also, when plated on a the M. smegmatis host at 37 degrees C, all of our dilutions of phage were able to grow plaques. In all of this we used the phage "Gumby" as a positive control, which allows us to determine if our phage samples were corectly created if both the samples and Gumby phage grew on the host bacteria. In our case, our phage dilutions were correctly made as we observed growth of plaques by all the phage.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesOur plaques are consistently about 1mm in diameter and are clear. This suggests we isolated a lytic phage in whihc the colonies of phage digested away the M.smegmatis host quickly and efficiently. Overall, our plaques are evenly spaced at a high concentration of similar appearances, meaning our phage has been well isolated.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.0X10^10pfu/ml
Date of SEA Lysate Titering Mar 22, 2016
Pitt Freezer Box# 40
Pitt Freezer Box Grid# D11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload