Mycobacterium phage Deshpanda
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| Detailed Information for Phage Deshpanda | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rohan Deshpande |
| Year Found | 2014 |
| Location Found | LaGrange, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.384844 N, 85.364302 E Map |
| Discovery Notes | The soil sample was selected 0.5-2.0 cm below the surface. The sample was taken in a shaded, wooded area near the base of a white pine. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Deshpanda grows within 24 hour when incubated at 37 degrees Celsius. Because of the early appearance of a clear center as well as the completely clear plaque after two days, I believe Deshpanda is a lytic phage. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaque of this phage is typically has a clear center along with a turbid ring. The size of the plaques are generally 0.7 cm in diameter when including the turbid areas. The inner, clear circle typically measures 0.2 cm. Long term incubation of Deshpanda in a cold environment and incubation at 37 degrees Celsius for more than two results in a completely clear plaque. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2014WEKUdeshpandaRD |
| SEA Lysate Titer | 9.0x10^8 |
| Date of SEA Lysate Titering | Oct 14, 2014 |
| Pitt Freezer Box# | 10 |
| Pitt Freezer Box Grid# | D5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
