Mycobacterium phage Diva17
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| Detailed Information for Phage Diva17 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zach Rub |
| Year Found | 2024 |
| Location Found | Boulder, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 1°C |
| GPS Coordinates | 40.008168 N, 105.266274 W Map |
| Discovery Notes | We isolated and increased the concentration of this phage by performing a series of experiments. The first experiment we performed was enrichment by collecting a hopeful soil sample containing the appropriate phage that would infect the m.Smeg bacteria we mixed and plated it with, and also to nourish and support the growth of the m.Smeg bacteria colonies in order for infection to occur illustrated in figure 1. We derived our soil sample by a creek in front of the CU Boulder Department of Geological Sciences. |
| Naming Notes | Diva17 was named as a "diva" due to its ambitious and strong ability to infect m.Smeg bacteria, create many plaques after several rounds of serial dilutions, and clearing complete plaque assay agar plates. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques measured between 2-3mm in diameter. DIVA17 is a Siphoviridae Lysogenic Phage undergoing the temperate life cycle as illustrated by its clear plaques with cloudy halo outlines. DIVA17 Electron Microscopy Reveals its classification as Siphoviridae Morphology. With characteristics such as a long non- contractile tail measured at 203.5 nm and a smaller head measured at 68.75 nm |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
