Arthrobacter phage DogEatDog
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| Detailed Information for Phage DogEatDog | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Ensley Alden, Phage Pop Hunterz, Phage Philes |
| Year Found | 2025 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.054637 N, 118.44514 W Map |
| Discovery Notes | Took out of a dog park which is next to a group of basketball courts, urban area, soil was moist Depth from which sample was taken (in cm): 15 cm Air temperature if sample collected outdoors (in degrees Celsius): 18.3oC Enrichment included incubating 15ml of soil with approx 40 ml of PYCa broth, plus we added 4ml of 48 hr culture containing Arthrobacter globiformis (B-2979), Arthrobacter atrocyaneous (NRRL-B-2883), Arthrobacter sulfureus (NRRL B1-4730) and Arthrobacter sp. (ATCC 21022). We then used a syringe filter to isolate possible phage particles from that enriched solution. We then performed 1 plate plaque assays for each host and determined if our lysate contained phages (incubated at 30oC for 48 hours) to elicit plaque formation for observation. Additional information of interest or note: Initially, this lysate contained phages that showed infectivity on A. golbi, A. sulf, and A. atro (when conducting a spot test). Upon further testing, the phage we isolated was not able to infect A. atro, so experimentation was done using A. globi and A. sulf. AL Quarter and Lab section: 1B Instructor: Dr. Tejas Bouklas Teaching Assistant: Pranava Jana Undergraduate Assistant(s): Ryan, Sara Team #1 Name: Phage Philes Team #1 Members: Aaliyah Tinoco, Ashley Lam, Sara Shahabi, Sean Verdijo Team #2 Name: PhagePopHunterz Team #2 Members: Eddy Zhu, Suah Choi, Ensley Alden, Athena Rodriguez, Jose Zamora |
| Naming Notes | It was collected from a dog park. DogEatDog was also inspired from the extravagant sayings “it’s a kill or be killed world” and “its a dog-eat-dog world”, much like how a phage behaves in its own environment. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaque Morphology Notes: pinpoint, consistently clear, minimal cloudiness around the edges. Description of plaque morphology: Clear pinpoint plaques Average size (and size range) diameter In mm: average 1.9 mm, ranged from 1 mm to 4 mm in diameter. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |