Mycobacterium phage Dovie
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| Detailed Information for Phage Dovie | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Jewel |
| Found By | Riley Burroughs |
| Year Found | 2022 |
| Location Found | Edmonton, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.942073 N, 85.621165 W Map |
| Discovery Notes | I collected my soil sample from my family farm. I retrieved it from a cow pasture next to a pond. I began by isolating phages from the soil sample and enriching the sample with Mycobacterium smegmatis. I filtered the enriched soil to prepare a spot plate. I diluted the filtered enrichment to isolate only one phage from my sample. I performed serial dilutions to purify the phage. I completed 6 of these dilution series to purify my phage. After achieving a 10 fold dilution, I created a one-plate lysate to gather all of the phage particles off of a webby plate to get the maximum number of healthy phages. I also made a ten-plate lysate to increase my phage titer and ensure enough lysate to isolate the phage DNA. The titer that I used was 7.7x10^7. The results from the DNA nanodrop were 81.7ng/uL. I further analyzed Dovie by performing restriction enzyme digest with gel confirmation. |
| Naming Notes | I am from Tompkinsville, KY and the one thing that my small hometown is known for is the Dovie burger. I named my phage after the restaurant Dovies. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques were lytic bullseye plaques. They had a transparent center with a small cloudy ring around them. They ranged in size from 0.3cm to 0.4cm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 7.7 x 10^7 |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | F9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
