Microbacterium phage Dozer
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Detailed Information for Phage Dozer | |
Discovery Information | |
Isolation Host | Microbacterium foliorum NRRL B-24224 |
Found By | Kayla Wheatley, Bill Gottschall |
Year Found | 2018 |
Location Found | Philadelphia, PA USA |
Finding Institution | University of the Sciences (now St. Joseph's Univ) |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 39.9468 N, 75.2071 W Map |
Discovery Notes | This sample was taken from an urban environment, about 12 feet from a building, and about 1 foot from foot traffic. This sample was moist and collected at a 6cm depth maximum. |
Naming Notes | I chose this name because it's my bulldogs name. He was named after a bulldozer, which deals with dirt and ground. I found his name fitting since the phage was isolated from a soil sample. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | EE |
Subcluster | -- |
Cluster Life Cycle | Lytic |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The phage completely cleared plates from 10^0 to 10^3. Webbed plates began at 10^4 and went through 10^6. The phage are lytic because the plaques were completely cleared, with clean edges, and a halo. Part of a pooled DOGEMS sample. Although a complete sequence wasn't obtained, Dozer was identified as an EE cluster using PCR. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | HVL: 1.32*10^9 pfu/mL |
Pitt Freezer Box# | 84 |
Pitt Freezer Box Grid# | D6 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |