Microbacterium phage Dozer
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| Detailed Information for Phage Dozer | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kayla Wheatley, Bill Gottschall |
| Year Found | 2018 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.9468 N, 75.2071 W Map |
| Discovery Notes | This sample was taken from an urban environment, about 12 feet from a building, and about 1 foot from foot traffic. This sample was moist and collected at a 6cm depth maximum. |
| Naming Notes | I chose this name because it's my bulldogs name. He was named after a bulldozer, which deals with dirt and ground. I found his name fitting since the phage was isolated from a soil sample. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The phage completely cleared plates from 10^0 to 10^3. Webbed plates began at 10^4 and went through 10^6. The phage are lytic because the plaques were completely cleared, with clean edges, and a halo. Part of a pooled DOGEMS sample. Although a complete sequence wasn't obtained, Dozer was identified as an EE cluster using PCR. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | HVL: 1.32*10^9 pfu/mL |
| Pitt Freezer Box# | 84 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |