Mycobacterium phage Electra
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| Detailed Information for Phage Electra | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Millicent Ronkainen |
| Year Found | 2014 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.883214 N, 86.525661 W Map |
| Discovery Notes | The soil sample was isolated from the top of a slight hill next to a road. The soil was damp and clay-like, and although it was surrounded by grass my sample did not come from soil with grass growing in it. The sample was taken from about 2.5 cm under the surface of the soil. |
| Naming Notes | Electra was an ancient Greek figure who plotted the death of her mother and step-father. Bacteriophage treat their bacterial hosts in a similar manner, using them to reproduce and then killing them to release new phage into the extracellular area. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | The majority of the plaques produced by the phage Electra do not appear to be lysogenic. Rather, they appear to be lytic because they create clear plaques. About 1.5% of the plaques on a plate seem to be lysogenic though, as they appear to be turbid. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Very clear, small, and well-defined plaques at and before 24 hours of incubation. Larger and fuzzier plaques after 24 hours of incubation. Electra exhibits two different plaque phenotypes. While the majority of plaques are small and clear with well-defined edges, about 1.5% of all plaques exhibit a turbid morphology. Through various tests, culminating in electron microscopy, I determined that this is merely a characteristic of Electra and is not caused by a mixed phage population. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2014WEKUelectraMR |
| SEA Lysate Titer | 1.8x10^9 |
| Date of SEA Lysate Titering | Oct 9, 2014 |
| Pitt Freezer Box# | 10 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |