Mycobacterium phage Encantado
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Detailed Information for Phage Encantado
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKathrine Gaiko
Year Found2015
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates36.985565 N, 86.448566 E Map
Discovery NotesA sunny humid day and the ground was damp enough for the soil to be compact. Obtained from behind a building next to a storage drain. Surrounded by little foliage, low to no amount of foot traffic, and low wind area.
Naming NotesChanged morphologies on me when almost to the end. Beautiful and consistent to begin with, but then changed and caused me a lot of trouble. Named after a Brazilian legendary creature that changed forms into a killer.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Lysogeny NotesChanged from 2 mm in diameter during the purification process and after my one plate lysate to 10 nm after my ten plate lysate.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesClear bullseye plaque or haloed plaque that changed sizes after ten plate lysate; went from large to very small 2 mm to 10nm. Incubated for 24 and 48 hours.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.1x10^7 pfu/ml
Date of SEA Lysate Titering Oct 28, 2015
Pitt Freezer Box# 27
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload