Mycobacterium phage Encantado
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Detailed Information for Phage Encantado | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Kathrine Gaiko |
Year Found | 2015 |
Location Found | Bowling Green, KY USA |
Finding Institution | Western Kentucky University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 36.985565 N, 86.448566 E Map |
Discovery Notes | A sunny humid day and the ground was damp enough for the soil to be compact. Obtained from behind a building next to a storage drain. Surrounded by little foliage, low to no amount of foot traffic, and low wind area. |
Naming Notes | Changed morphologies on me when almost to the end. Beautiful and consistent to begin with, but then changed and caused me a lot of trouble. Named after a Brazilian legendary creature that changed forms into a killer. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Lysogeny Notes | Changed from 2 mm in diameter during the purification process and after my one plate lysate to 10 nm after my ten plate lysate. |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | Clear bullseye plaque or haloed plaque that changed sizes after ten plate lysate; went from large to very small 2 mm to 10nm. Incubated for 24 and 48 hours. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 3.1x10^7 pfu/ml |
Date of SEA Lysate Titering | Oct 28, 2015 |
Pitt Freezer Box# | 27 |
Pitt Freezer Box Grid# | E4 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |