Mycobacterium phage FlagellaFella
Add or modify phage thumbnail images to appear at the top of this page.
Know something about this phage that we don't? Modify its data.
| Detailed Information for Phage FlagellaFella | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Meredith Gail Feller |
| Year Found | 2021 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.982354 N, 86.447205 W Map |
| Discovery Notes | My soil sample was enriched and spot plated on a bacterial lawn. Three serial dilutions were performed, but the plaques still varied in size. A big and small test was performed to ensure isolation of a single phage, and then a webby plate was plated for my one plate lysate. That lysate was then titered, collected, serial diluted, archived, and plated for my ten plate lysate. The first plating was unsuccessful, but a second performance of the plating was. The lysate was collected, filtered, serial diluted, and examined under the EM. The same lysate was also used for DNA isolation, which enabled restriction enzyme digest and gel electrophoresis to be run twice with two sets of enzymes. |
| Naming Notes | I wanted to make a play on my last name (Feller) that was biology-related and rhymed. FlagellarFeller was my original idea, but exceeded the character count limit. FlagellaFella was the best satisfaction of my own stipulations for the name that met the character limit. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My plaques were usually clustered together, with it being rare to find a single plaque who was not touching the boundary of another plaque. The plaques were about 4 mm in diameter with a roughly 1 mm in diameter clear bullseye in the center of their turbid halo. The plaques did not change in nature over time, but changed in size as the original plaques were all so squished together that it was impossible to distinguish the boundary of one from another. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.06 x 10^9 |
| Pitt Freezer Box# | 137 |
| Pitt Freezer Box Grid# | G8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
