Mycobacterium phage FourStarNutty
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Detailed Information for Phage FourStarNutty
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAllyson Roberts
Year Found2012
Location FoundBaltimore, MD USA
Finding InstitutionJohns Hopkins University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesThe soil gathered was taken from a community garden of Johns Hopkins University. It was collected at approximately 10:30am, from approximately 4cm beneath the surface. The soil was fairly dry and was not directly next to or surrounding any live plants.
Naming NotesAfter coming into the lab outside of my class on a Friday afternoon, I developed a stronger bond with my professor. Both of us are fairly sassy, and we bantered back and forth. By the end of the day, my professor made a comment about how he was going to have a "Four-Star Nutty." Apparently it refers to some sort of fit, and was a popular phrase "back in the day." While Google doesn't return a single result defining the term as my professor had, and I'm not sure the phrase is legitimate at all, it seemed like a fitting name for my temperamental and aggressive phage (while also poking fun at my professor).
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Cluster Life CycleTemperate
Annotation StatusNot sequenced
Plaque NotesThe plaques are of a bulls-eye morphology, and typically appear to be approximately 3-5mm in diameter (however, if two or more plaques merge together, they can sometimes appear to be a single, larger plaque). The plaques are often fairly aggressive, typically either completely covering a plate with plaques or having no plaques on a plate.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator FourStarNutty
SEA Lysate Titer 8.1 x 109 pfu/mL
Date of SEA Lysate Titering Oct 11, 2012
Pitt Freezer Box# 13
Pitt Freezer Box Grid# B1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload