Mycobacterium phage Funbox
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| Detailed Information for Phage Funbox | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Theresa Wong and Jason Ho |
| Year Found | 2008 |
| Location Found | San Diego, CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.87851 N, 117.23864 W Map |
| Discovery Notes | Taken from soil, depth 1 cm, 76 deg F, near a Eucalyptus forest on UCSD campus, 5ft from trail within Eucalyptus forest, surrounded by newly sprouted green plants, slightly damp almost black soil looser firmness, little to no human activity. |
| Growth Notes | It’s interesting to note that when plated out in high titers, Fun Box will produce small, clear plaques. In lower titers, the plaques are larger, though still not very large. For the most part, these plaques are all small and clear. Incubating them for longer periods may result in a turbid halo surrounding the clear plaques. However, on the spot test, Fun Box would produce large plaques with multiple layers or rings. The center of the plaque was turbid, while the outer rings would be clear. I concluded that Fun Box somehow “knows” to remain lysogenic when in the presence of a high titer, or concentration of itself. When there is a lower concentration of Fun Box present, such as in the outer rings of the plaque, the phage would revert to the lytic phase, killing the bacteria, which might explain why the center of the plaque is turbid while the outer rings were clear in the spot test. It also might explain the phage’s behavior on the plates when they remained smaller in high titer plates. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Depends on titer. Generally small in high titers; around 1-2 mm diameter. Clear centers, sometimes with turbid halo if allowed to incubate over 48 hours at 37 deg C. Larger plaques formed at lower tiers, usually 3 mm in diameter. Can grow up to 4-5 mm in diameter if incubated over 48 hours. Otherwise, same plaque morphology as described above when incubated at similar conditions. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Funbox |
| SEA Lysate Titer | 1.30e11 |
| Pitt Freezer Box# | 6 |
| Pitt Freezer Box Grid# | 8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
