Mycobacterium phage Gamesh
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| Detailed Information for Phage Gamesh | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ahmad Alsaed |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 45.39107 N, 75.6377 W Map |
| Discovery Notes | Phage Gamesh was discovered in a corn plot at Kilborn’s Community Garden in dark, loamy soil at an ambient temperature of 24 °C. Phage Gamesh was discovered in a corn plot at Kilborn’s Community Garden in dark, loamy soil at an ambient temperature of 24 °C. Genomic DNA was sequenced on a ONT MinION, R10.4.1 chemistry. The sequence assembled into one contig of 68,389 bp with 99% query cover, 99.71% identity to Mycobacterium phage Timmi(Cluster B1). This sequence is complete based on the size of other Cluster B1 phages. |
| Naming Notes | Phage Gamesh is named after the Mesopotamian hero Gilgamesh, inspired by my interest in Mesopotamian legends and mythology. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | On M. smegmatis mc²155 at 37 °C, Gamesh forms small (~1–2 mm), round plaques with clear centers and a faint, turbid halo, giving a subtle bullseye appearance. Plaques are well-defined and fairly uniform across dilutions, with no obvious concentric rings or plaque “filling in” even after extended incubation (24–48 h). I did not observe mixed turbid vs. clear morphologies on the same plate. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
