Mycobacterium phage Gardeno
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| Detailed Information for Phage Gardeno | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ntuthuko Mbhense |
| Year Found | 2013 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.817897 N, 30.942771 W Map |
| Discovery Notes | Soil sampled was enrioched with Mycobacterium smegmatis + phage buffer, incubated at 37 degrees celcius for ~24 hours. 1 mL of liquid was filtered through a syringe filter, added to M. smegmatis + molten agar and incubated for 24 hours at 37 degrees celcius. An isolated plaque was picked diluted in phage buffer, added to M. smegmatis + molten agar, plated out and incubated. Purification: The plate was flooded with 10 mL phage buffer, left for hours and the liquid filtered and the lysate was prepared. |
| Naming Notes | The phage was isolated from garden soil in UKZN, Westville Campus. "Gardeno" comes from the phage source. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Clear 1-2 mm plaques with entire or smooth edges. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |