| Detailed Information for Phage Ghobes | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jonathan Ghobrial |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.445208 N, 79.961812 W Map |
| Discovery Notes | The soil sample from which the phage was discovered is from a small garden outside of Panther Hall at the University of Pittsburgh, Pittsburgh, PA. It was 75 degrees, sunny, and humid outside. The soil was very moist and a dark brown color. |
| Naming Notes | The phage was named after me. Ghobes is my nickname. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 27, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2485 |
| Genome length (bp) | 45285 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCCGAGGTA |
| GC Content | 65.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DA |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to View |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The phage had turbid plaques with two plaque morphologies. The plaque morphologies were 2.5mm and 1mm in diameter. Both morphologies were present at any given time. |
| Morphotype | Siphoviridae |
| Number of Genes | 59 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGhobes_1 Ghobes_2 Ghobes_3 Ghobes_4 Ghobes_5 Ghobes_6 Ghobes_7 Ghobes_8 Ghobes_9 Ghobes_10 Ghobes_11 Ghobes_12 Ghobes_13 Ghobes_14 Ghobes_15 Ghobes_16 Ghobes_17 Ghobes_18 Ghobes_19 Ghobes_20 Ghobes_21 Ghobes_22 Ghobes_23 Ghobes_24 Ghobes_25 Ghobes_26 Ghobes_27 Ghobes_28 Ghobes_29 Ghobes_30 Ghobes_31 Ghobes_32 Ghobes_33 Ghobes_34 Ghobes_35 Ghobes_36 Ghobes_37 Ghobes_38 Ghobes_39 Ghobes_40 Ghobes_41 Ghobes_42 Ghobes_43 Ghobes_44 Ghobes_45 Ghobes_46 Ghobes_47 Ghobes_48 Ghobes_49 Ghobes_50 Ghobes_51 Ghobes_52 Ghobes_53 Ghobes_54 Ghobes_55 Ghobes_56 Ghobes_57 Ghobes_58 Ghobes_59 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX557278 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 2 |
| Pitt Freezer Box Grid# | E11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
