Gordonia phage GoFish
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| Detailed Information for Phage GoFish | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Nitya Reddy |
| Year Found | 2018 |
| Location Found | Erie, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.1489 N, 80.1327 W Map |
| Discovery Notes | I collected the soil sample near Lake Erie. It was collected in a little park near the grills where people cook. The soil was pretty dry, but there were many trees around. The grass around where I collected my soil sample was yellow. After completing the direct isolation and spot test protocols, I got 6 positive clearings for my spot test. I chose one of those clearings to start my plaque purification. |
| Naming Notes | I came up with the name because I collected the soil sample I isolated my phage from near a lake. When I think of lakes, I think of fish, so I named the phage GoFish. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | I performed an alternative spot titer on phage GoFish. I waited for mesas to form, and then I streaked the mesa out to dilute it further. Individual colonies formed in the streaked plate. Since individual colonies formed, there is a possibility that phage GoFish is capable of forming lysogens which also means phage GoFish is possibly a temperate phage. However, I was not able to do further testing to confirm whether phage GoFish is a temperate or lytic phage. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaque I chose, the edges were relatively smooth and it was a decent sized plaque. For the most part, the plaque was pretty circular. The clearing is completely clear and about 1-1.5 cm big. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 90 |
| Pitt Freezer Box Grid# | B12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |