Gordonia phage Granola
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Detailed Information for Phage Granola
Discovery Information
Isolation HostGordonia terrae 3612
Found ByMeera Garg
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.44628 N, 79.95314 W Map
Discovery NotesThe soil was collected on an overcast morning, and the temperature was around 77 degrees F. The soil was mostly dry, and it was adjacent to bushes and underneath mulch. The soil was transported for 2 minutes at outside temperature and it remained in a backpack for approximately 1 hour before direct isolation.
Naming NotesOn the direct isolation and spot test plates, the plaques from the phage gave a speckled appearance, which reminded the finder of granola and its various components. Also, granola is very near and dear to the finder's heart.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesFor plaque purification, Granola was grown and incubated for 3 days with 1x top agar on a PYCa plate and Gordonia terrae. The plaques are completely clear with well-defined edges. Plaque sizes are small and round, ranging between 1-2mm in diameter. Considering that lytic phages generally produce clear plaques, and temperate phages usually have cloudy plaques, Granola is currently hypothesized to be a lytic phage.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 90
Pitt Freezer Box Grid# C1
Available Files
Plaque PictureDownload
EM PictureDownload