Mycobacterium phage GravelGirlies
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| Detailed Information for Phage GravelGirlies | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Marissa Stevenson and Ellie Sadlow |
| Year Found | 2023 |
| Location Found | North Andover, MA United States of America |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.3958 N, 71.719 W Map |
| Discovery Notes | All sampling was done at GPS coordinates 42°39’58’’ N 71°7’19” W. This was to the left of the dirt parking lot between Ash Center and the Soccer Field. The sample was taken in slightly in front and to the left of a metal pole sticking out of the ground near the gate across from the dumpster. The sample was taken shortly after it had rained. A latex glove was used to remove the top layer of mud. The sample was collected about 3 inches down. The sample was immediately put put into a clean plastic sandwich bag and sealed. The sample was collected at 8:12pm on September 17th. The sample was thoroughly moisturized due to the earlier rain. It was more mud then dirt. The ambient temperature for the day was 68 degrees Fahrenheit with 69% humidity on a mostly cloudy day. The sample was collected before isolation, and was stored in a fridge. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | In the generation one plaques they all came out very cloudy and white. In both the 10^-1 and 10^-3 plates it looked as though there was some sort of plaque but in this generation we decided to pick plate 10^-3 since it had the most isolated plaque. In 10^-3 there was one distinct plaque that we counted. This plaque was not fully clear but also not as cloudy as the rest of the plate. There is no clear ring around the plaque, it is just one transparent circle. The edges on this plaque were unclear from the top agar and it looks flat to the agar. There were little white spots all over the plate. The plaque that we circled in this lab was about 0.3cm big. The dilution that seemed to create the most plaques in this generation would have been the 10^-1 dilutions. The three plates all looked pretty similar; they all were cloudy and opaque. Generation one out of the five generations created the most plaques. Each time we conducted this lab the plates had similarities. Most of them were cloudy and hard to see through and they all had very little white dots/spots all over them. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 182 |
| Pitt Freezer Box Grid# | B10 |
| Available Files | |
| Plaque Picture | Download |
