Microbacterium phage GreenRat
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Detailed Information for Phage GreenRat
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByD Vohra, K Pousland, A Tringalu, N Ricketts
Year Found2025
Location FoundAmherst, MA USA
Finding InstitutionUniversity of Massachusetts Amherst
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates42.389724 N, 72.529528 W Map
Discovery NotesThis phage sample was collected in between Thompson Hall and South College buildings. Most of the surrounding soil was covered in snow with only a small patch of soil being visible.The soil was damp.
Naming NotesThe name we chose for our phage is GreenRat. We got inspiration for this name from our first lab procedure. When we had to get soil collections, D Vohra had a green piece of fertilizer in her soil. From there, we took the word green. The Rat part of the word comes from the idea that the phage has failed us many times. We’ve had to do both isolation and purification multiple times and it has left us somewhat annoyed- just like a rat.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque Notes3 different morphologies;
First type of plaque was very small with an extremely small center, clear (about 2.5-3mm in diameter)

For second type, there were 2 separated plaques that all look to have formed a bullseye around a smaller “dot” than the third type, formed a one ring outside of the center (about 6.5mm in diameter)

Third type formed with two conjoined plaques, one right in the middle, and one a the very side of the plate; plaques were somewhat cloudy, the center was bigger than type two, a double ring formed outside of the center (about 9mm in diameter)
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives