Mycobacterium phage Gustavson
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| Detailed Information for Phage Gustavson | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ben Wilhelm |
| Year Found | 2024 |
| Location Found | Boulder, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.00542 N, 105.264739 W Map |
| Discovery Notes | The soil sample was collected on February 6, 2025, from a shaded, mulched area with damp soil and a thin layer of wood chips. Enrichment was carried out by incubating the soil with M. smegmatis for 48 hours at 37°C. Initial plaque assays revealed the presence of potential phage activity, leading to successive rounds of purification. Following initial purification and spot testing, multiple plaque morphologies were observed, suggesting a mixed phage population. |
| Naming Notes | The phage was named Gustav to give a distinct identity to the isolate. The name reflects a personal choice and carries no direct biological implication. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Phage Gustav displays morphology characteristic of siphoviruses, with an icosahedral capsid and a long, flexible, non-contractile tail, as seen in EM images. While this structural class is often associated with temperate phages, plaque assay results suggest that Gustav is strictly lytic. It consistently produces clear, well-defined plaques across multiple dilutions, including web pattern plates indicative of high phage activity. No turbid halos or signs of lysogeny were observed, and negative control plates confirmed the absence of contamination. Together, these findings indicate that Gustav is a lytic siphovirus, capable of efficient and complete lysis of its Mycobacterium smegmatis host. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |