Mycobacterium phage HaiitsPooja
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Detailed Information for Phage HaiitsPooja
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPooja Naidugari
Year Found2016
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature78°C
GPS Coordinates36.986806 N, 86.454083 W Map
Discovery NotesI found this on WKU's campus. It was found in some moist soil when the climate was pretty dry (78 degrees). The site was hidden behind some trees in the shade. It included a lot of mulch but the actual soil underneath was moist.
Naming NotesI named my phage Haiitspooja because it is a childhood name and because it was my user name when i was creating my social media.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Lysogeny NotesOnce my phage was purified, I ended up having a clear plaque, which implies my phage underwent the lytic cycle.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesMy plaques were clear and cloudy. The first plate had a mixture of both clear and cloudy plaques and then once purified it turned out to be a clear plaque, meaning that it was probably lytic. My final purified plaque was about 0.5 cm in diameter (the clear plaque).
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUhaiitspoojaPN
SEA Lysate Titer 3.5 x 10^9 pfu/mL
Date of SEA Lysate Titering Nov 3, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload