Mycobacterium phage HeWhoMustBNamd
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| Detailed Information for Phage HeWhoMustBNamd | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rebecca Camacho |
| Year Found | 2014 |
| Location Found | Baltimore , MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.331117 N, 76.618761 W Map |
| Discovery Notes | Retrieval Date: 08/27/2014 at 3:50 p.m. Weather: slightly humid at 88° F Soil Conditions: slightly moist from rain Soil Depth in Ground: about 5.5 cm deep Location on Campus: Outside AMR II near construction Features of Site: urban, slight slope, next to a cemented pathway and small parking lot, minimal foot traffic since hidden almost, no trees near spot, vent nearby, light post nearby, water near in storm drain |
| Naming Notes | The name for this phage comes from the fact that the it put up a good fight from start to finish especially during the genomic DNA purification phase in which it refused to go through the syringe and into the column. It was at that point, and three broken syringes later, that I realized that it resembled the character of Lord Voldemort from Harry Potter since he too never rested and continued to wreak havoc. I also had a very hard time naming my phage and spent many weeks thinking about it so I thought the name fit both aspects of it's creation. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | clear small to plaques with medium clear bullseye, 650 micron head with a 1.9 micron tail, bulls-eye in shape, 140 plaques average per plate with 179 plaques at highest titer |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.79E10 |
| Date of SEA Lysate Titering | Oct 28, 2014 |
| Pitt Freezer Box# | 14 |
| Pitt Freezer Box Grid# | A4 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |