| Detailed Information for Phage Heather | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Goralski, Stephen & Markward, Michelle |
| Year Found | 2018 |
| Location Found | Burtonsville, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.111218 N, 76.932426 W Map |
| Discovery Notes | Taken from the front lawn of a home on a cloudy date at 24.4°C . |
| Naming Notes | Named after the class movie Heathers and renown musical Heathers: The Musical |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2280 |
| Genome length (bp) | 41238 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCCGGCCCCA |
| GC Content | 62.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BB |
| Subcluster | BB2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Turbid center with average diameter of 8mm. Halo ring forms around. |
| Morphotype | Siphoviridae |
| Number of Genes | 57 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewHeather_1 Heather_2 Heather_3 Heather_4 Heather_5 Heather_6 Heather_7 Heather_8 Heather_9 Heather_10 Heather_11 Heather_12 Heather_13 Heather_14 Heather_15 Heather_16 Heather_17 Heather_18 Heather_19 Heather_20 Heather_21 Heather_22 Heather_23 Heather_24 Heather_25 Heather_26 Heather_27 Heather_28 Heather_29 Heather_30 Heather_31 Heather_32 Heather_33 Heather_34 Heather_35 Heather_36 Heather_37 Heather_38 Heather_39 Heather_40 Heather_41 Heather_42 Heather_43 Heather_44 Heather_45 Heather_46 Heather_47 Heather_48 Heather_49 Heather_50 Heather_51 Heather_52 Heather_53 Heather_54 Heather_55 Heather_56 Heather_57 — tRNA Heather_58 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK686069 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.1x10^10 pfu/mL |
| Pitt Freezer Box# | 83 |
| Pitt Freezer Box Grid# | F7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
