Mycobacterium phage Hope3000
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| Detailed Information for Phage Hope3000 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Juliana McCormick / Ryan Doss |
| Year Found | 2020 |
| Location Found | Las Vegas, NV United States of America |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 27°C |
| GPS Coordinates | 36.1099 N, 115.143 W Map |
| Discovery Notes | The Environmental Sample was collected from a potted plant obtained in the laboratory at the University of Nevada Las Vegas. The soil was collected from the middle of the plant's root base. The soil was black, dry and encompassed by roots. Small amounts of vermiculite were present in the soil. Direct Isolation was performed and Phages were extracted from the solid environmental sample, enriched with broth 7H9, shaken at 37℃ for 1.5 hours incubation time, and centrifuged for 5 minutes at 4000 g. |
| Naming Notes | Hope 3000 was selected as the name of the phage due to a book called The Perfect Predator. The book was based on a true story where the authors husband fell extremely ill and was treated with phage therapy. When all other treatments failed phage therapy prevailed. The name Hope 3000 represents a phage that may be useful to science and beneficial to people. The latter 3000 part of the name is for the tradition that has been kept at the University of Nevada Las Vegas. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques were 2mm in size, with low turbidity, clear and smooth edges. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 120 |
| Pitt Freezer Box Grid# | H4 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |