Streptomyces phage IceyHot
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Detailed Information for Phage IceyHot
Discovery Information
Isolation HostStreptomyces scabiei RL-34
Found ByKristina Atanasoff and Laiyana Kabir
Year Found2018
Location FoundBaltimore, MD USA
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates39.248204 N, 76.707039 W Map
Discovery NotesThe soil from which the phage was isolated was brown and clumpy, located under a rose bush in front of the UMBC dining hall (aka D-Hall). The weather was humid and warm at time of collection.
Naming NotesOur phage demonstrates a temperature-related gradient in plaque formation when grown on incubated plates; the name "IceyHot" humorously marks this unique trait.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterBI
Subcluster--
Cluster Life CycleLytic
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPlaques of the phage grew very slowly, often only appearing after 72 hours in the incubator. Plaques were circular and small, with a diameter of approx. 1 mm. Plaques were very turbid; if the phage lysed a plate out, the plate would often still appear "cloudy" with clearer plaques within as more phages lysed bacteria.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.7x10^8 pfu/mL
Pitt Freezer Box# 83
Pitt Freezer Box Grid# G1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload