Mycobacterium phage Ironfist
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| Detailed Information for Phage Ironfist | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Josef Mang and Ahmad Elmohtaseb |
| Year Found | 2016 |
| Location Found | Fairfax, VA USA |
| Finding Institution | George Mason University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.8316 N, 77.306916 W Map |
| Naming Notes | Ironfist originated from how this was the only phage to found among the 5 samples that originally were tested. Some prospective phages, we're slow growing and ultimately ceased to culture after purification, resulting in this being the only viable phage found. It is very hardy and easily cultured. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Two plaque morphologies, small and medium, presented themselves when plated with M. smegmatis, and this phage was isolated from the small plaques. Plaques measured approximately less than 1mm across when observed during the first round of purification. When plated during the second round of purification, the plaques increased to around 2 to 3mm. Almost perfectly spherical morphological were observed and we're completely transparent. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 46 |
| Pitt Freezer Box Grid# | G1 |
| Available Files | |
| Plaque Picture | Download |