Mycobacterium phage Ironfist
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Detailed Information for Phage Ironfist
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJosef Mang and Ahmad Elmohtaseb
Year Found2016
Location FoundFairfax, VA USA
Finding InstitutionGeorge Mason University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.8316 N, 77.306916 W Map
Naming NotesIronfist originated from how this was the only phage to found among the 5 samples that originally were tested. Some prospective phages, we're slow growing and ultimately ceased to culture after purification, resulting in this being the only viable phage found. It is very hardy and easily cultured.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesTwo plaque morphologies, small and medium, presented themselves when plated with M. smegmatis, and this phage was isolated from the small plaques. Plaques measured approximately less than 1mm across when observed during the first round of purification. When plated during the second round of purification, the plaques increased to around 2 to 3mm. Almost perfectly spherical morphological were observed and we're completely transparent.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 46
Pitt Freezer Box Grid# G1
Available Files
Plaque PictureDownload