Mycobacterium phage Isee1101
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|Detailed Information for Phage Isee1101|
|Isolation Host||Mycobacterium smegmatis mc²155|
|Found By||Eli Silvert|
|Location Found||Providence, RI USA|
|Finding Institution||Brown University|
|Program||Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science|
|From enriched soil sample?||Yes|
|Isolation Temperature||Not entered|
|GPS Coordinates||41.825628 N, 71.404837 W Map|
|Discovery Notes||This phage was found in the flower pot to the left of the Rockefeller Library front stairs when facing them. It was in soil under white and red flower in the planter.|
The sample was collected on September 8, 2016.
The sample was 0 to 3 cm below the surface of the soil.
It was collected in air temperature of 79 degrees Fahrenheit, after it had recently rained.
|Naming Notes||Isee1101 is named after the galaxy IC 1101. There are two similarities between my phage and this galaxy:|
1. Both are large for their kind. This is a cluster C phage, which have a huge genome for phage - around 150,000 base pairs. Likewise, IC 1101 is the largest known galaxy.
2. Both were fairly industrious and productive in the beginning, and then became more stagnant as time went on. To explain in the context of my phage, I was very quick to find a phage, isolate it, and create a large lysate. However, I became less productive in the later stages of working with my phage--in extracting DNA and restriction enzyme work, just by chance. Likewise, IC 1101 was very productive when it was relatively young. It created stars, merged with other galaxies, altogether growing rapidly. However, in its later stages it has become far less industrious. It's growth slows because it uses up the materials necessary to create stars and because other galaxies get further apart.
I also chose this name for the somewhat humorous dichotomy between a microscopic phage and one of the largest things in the universe (spanning 60 million light-years in diameter).
|Genome length (bp)||Unknown|
|Character of genome ends||Unknown|
|Fasta file available?||No|
|Cluster Life Cycle||Lytic|
|Annotation Status||Not sequenced|
|Plaque Notes||Fairly circular--although not perfect--plaques less than one centimeter in diameter. Plaques are translucent, suggesting temperate phage.|
|Has been Phamerated?||No|
|Uploaded to GenBank?||No|
|GenBank Accession||None yet|
|Refseq Number||None yet|
|Archiving status||Not in Pitt Archives|