Mycobacterium phage Isee1101
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Detailed Information for Phage Isee1101
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEli Silvert
Year Found2016
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.825628 N, 71.404837 W Map
Discovery NotesThis phage was found in the flower pot to the left of the Rockefeller Library front stairs when facing them. It was in soil under white and red flower in the planter.
The sample was collected on September 8, 2016.
The sample was 0 to 3 cm below the surface of the soil.
It was collected in air temperature of 79 degrees Fahrenheit, after it had recently rained.
Naming NotesIsee1101 is named after the galaxy IC 1101. There are two similarities between my phage and this galaxy:
1. Both are large for their kind. This is a cluster C phage, which have a huge genome for phage - around 150,000 base pairs. Likewise, IC 1101 is the largest known galaxy.
2. Both were fairly industrious and productive in the beginning, and then became more stagnant as time went on. To explain in the context of my phage, I was very quick to find a phage, isolate it, and create a large lysate. However, I became less productive in the later stages of working with my phage--in extracting DNA and restriction enzyme work, just by chance. Likewise, IC 1101 was very productive when it was relatively young. It created stars, merged with other galaxies, altogether growing rapidly. However, in its later stages it has become far less industrious. It's growth slows because it uses up the materials necessary to create stars and because other galaxies get further apart.
I also chose this name for the somewhat humorous dichotomy between a microscopic phage and one of the largest things in the universe (spanning 60 million light-years in diameter).
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Cluster Life CycleLytic
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesFairly circular--although not perfect--plaques less than one centimeter in diameter. Plaques are translucent, suggesting temperate phage.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives