Microbacterium phage Isperia
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Detailed Information for Phage Isperia
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-24275
Former namesTriph
Found ByFallyn, Alex
Year Found2023
Location FoundCullowhee, NC, NC United States
Finding InstitutionWestern Carolina University
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates35.31032 N, 83.18738 W Map
Discovery NotesNear the creek by the WCU freshman lot. Relatively moist dirt.
Naming NotesNamed after a Magic: the Gathering card.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 14, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage20271
Genome length (bp)32498
Character of genome endsCircularly Permuted
End Determination NotesThere are no obvious buildups of read starts anywhere in the sequencing data set, as well as no clear differences in coverage throughout the genome that would be indicative of defined ends. Ergo, this cluster's phages are considered to have circularly permuted genomes.
GC Content68.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterGJ
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionGettysburg College
Annotation StatusBeing Annotated (Expected completion by 5/1/2025)
Plaque NotesSome of the phages' tailswas abnormally long. Others were about average size.
MorphotypeSiphoviridae
Number of Genes51
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.2*10^10
Pitt Freezer Box# 175
Pitt Freezer Box Grid# F7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload