Mycobacterium phage Jorianna
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| Detailed Information for Phage Jorianna | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gianna Gaeta and Jordan Lavoie |
| Year Found | 2023 |
| Location Found | North Andover, MA United States of America |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.66778 N, 71.12162 W Map |
| Discovery Notes | The sample was properly collected at gps coordinates 42.66778°N, 71.12162°W. Initially, the area above the sampling point was excavated by a person using a glove in hopes of avoiding contamination. After digging about four inches below ground level, the sample was collected and stored in a sterile tube, which was then sealed inside a plastic ziploc bag. In order to ensure proper and accurate results, initials JL and GG were written on the bag, along with the date and time in which the sample was collected, which was 9/18/23 at around 3pm. On this date, the temperature was mid 60s, with consistent precipitation throughout the whole day. Additionally, the soil was extremely damp at the time of collection due to the environmental conditions. The dew point averaged 62.4 degrees fahrenheit, and the max wind speed was 8mph. Although the dew point doesn’t directly affect the soil itself, it promotes environmental conditions in which various fungus and other microbiological processes are able to thrive. |
| Naming Notes | Named for the two students who found and isolated the phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | After the preparation of generation two plaque assays were completed, the results were analyzed. In this generation there was a total of the plaques present, as the 10-3 plate yielded three plaques and the 10-1 and 10-5 plaques yielded zero plaques. The largest plaque, which was around 0.3cm in diameter, was used for observation. Although the plates were not completely heterogenous, the plaques showed more similarities in this generation than they did in generation one. All of the plaques were similar in size, and they all had a cloudy appearance with cloudy edges. These results were somewhat surprising, as the plaques were only present in the 10-3 dilution. On the other hand, the increasing similarities between the plaques were expected and desirable. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 182 |
| Pitt Freezer Box Grid# | B12 |
| Available Files | |
| Plaque Picture | Download |
