Mycobacterium phage Jorianna
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Detailed Information for Phage Jorianna
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGianna Gaeta and Jordan Lavoie
Year Found2023
Location FoundNorth Andover, MA United States of America
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.66778 N, 71.12162 W Map
Discovery NotesThe sample was properly collected at gps coordinates 42.66778°N, 71.12162°W. Initially, the area above the sampling point was excavated by a person using a glove in hopes of avoiding contamination. After digging about four inches below ground level, the sample was collected and stored in a sterile tube, which was then sealed inside a plastic ziploc bag. In order to ensure
proper and accurate results, initials JL and GG were written on the bag, along with the date and time in which the sample was collected, which was 9/18/23 at around 3pm. On this date, the
temperature was mid 60s, with consistent precipitation throughout the whole day. Additionally, the soil was extremely damp at the time of collection due to the environmental conditions. The
dew point averaged 62.4 degrees fahrenheit, and the max wind speed was 8mph. Although the dew point doesn’t directly affect the soil itself, it promotes environmental conditions in which various fungus and other microbiological processes are able to thrive.
Naming NotesNamed for the two students who found and isolated the phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesAfter the preparation of generation two plaque assays were completed, the results were analyzed. In this generation there was a total of the plaques present, as the 10-3 plate yielded three plaques and the 10-1 and 10-5 plaques yielded zero plaques. The largest plaque, which was around 0.3cm
in diameter, was used for observation. Although the plates were not completely heterogenous, the plaques showed more similarities in this generation than they did in generation one. All of
the plaques were similar in size, and they all had a cloudy appearance with cloudy edges. These results were somewhat surprising, as the plaques were only present in the 10-3 dilution. On the other hand, the increasing similarities between the plaques were expected and desirable.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 182
Pitt Freezer Box Grid# B12
Available Files
Plaque PictureDownload