| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Saransh Gothi and John H. Alarcon |
| Year Found | 2016 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 27°C |
| GPS Coordinates | 38.645708 N, 90.311579 W Map |
| Discovery Notes | The soil sample from which the phage was isolated was collected at the Burning Kumquat Garden on the South 40 of Washington University in St. Louis. The soil was loamy, moderately moist, and rich in organic matter (compost had been mixed into the soil recently). Soil was collected at a depth of 8 cm. |
| Naming Notes | We named our phage JustBecause just because |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 25, 2017 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 129 |
| Genome length (bp) | 184281 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1043 bp |
| End Determination Notes | The right end of this genome was clearly defined by a read start buildup on one edge of a double-coverage region. The left end was more gradual, and so an approximate left end was chosen near the other edge of the read buildup. |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BM |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Small plaques formed by phage had a clear centre surrounded by turbidity and further surrounded by a ring of clearing. Plaques were 1-2 mm in diameter. |
| Number of Genes | 325 |
| Number of tRNAs | 14 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH744418 |
| Refseq Number | None yet |
| Published in Paper | Shaffer et al 2019, Microbiology Resource Announcements: Complete Genome Sequences of Streptomyces lividans Bacteriophages Satis and JustBecause |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
2.4x10^7 pfu/mL |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
