Mycobacterium phage Justus
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Detailed Information for Phage Justus | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Kristin Stockdale |
Year Found | 2015 |
Location Found | Bowling Green, KY USA |
Finding Institution | Western Kentucky University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 36.98 N, 86.45 W Map |
Discovery Notes | I found the phage by extracting soil from the surface of an area that had decaying leaves, branches, and plants. The phage was very easy to grow and took between 24-48 hours. The plaques were large and easy to see even though it was turbid. I never had trouble purifying or growing the plaques. |
Naming Notes | The name came from a mountain I hike last summer in Georgia. I hiked over ten mountains that week, but this one was the most biologically diverse one I saw. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Lysogeny Notes | It is characterized by the lytic cycle. |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | Turbid plaques that had a 24 to 48 hour incubation period in 30˚ conditions. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 5.74E9 |
Date of SEA Lysate Titering | Oct 19, 2015 |
Pitt Freezer Box# | 27 |
Pitt Freezer Box Grid# | F1 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |