Mycobacterium phage KROO
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| Detailed Information for Phage KROO | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Austin Roblyer |
| Year Found | 2010 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.787897 N, 86.103644 W Map |
| Discovery Notes | Next to Voorhees Hall on Hope College campus, in front of the left leg of the Voorhees sign. Next to pink flowers to the right of the area where the sample was taken. Taken from dry, sandy soil close to foot traffic within feet of brick building. Air temperature at 63 degrees F. Sample taken at 6 to 7 centimeters in the soil. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Twenty-four hours after initial plating of KROO, the plaque size varied between one or three millimeters in width, with most plaque sizes leaned towards the lower end of the size spectrum. The smaller plaques were usually clustered near a single larger plaque size on the plate. Each of the larger, three millimeter plaques were not positioned near another of a similar size. By the end of forty-eight hours, all the plaques had grown to an approximate uniform size of four to five millimeters in width, with some morphology based evidence suggesting they were variable sizes before. After forty-eight hours had passed, the growth rate of the plaques slowed down drastically, continually growing but at a much reduced pace. The plaque morphology shifted slightly over the time period described above. During the first twenty-four hours, each individual plaque had a clear central area, usually half a millimeter less than the actual width of the plaque. This area was almost a perfect circle. The extra half millimeter was taken up by a developing fuzzy, turbid area which surrounded the clear center, forming a jagged outer edge that slowly faded away. The smaller the plaque, the less turbid the edges were (i.e. the one millimeter plaques looked like a clear dot on the bacterial lawn, whereas the three millimeter plaques looked more like the description above). After forty-eight hours, the morphology entered a different developmental stage. The clear center was still the prominent spot on the plaque, and had grown a little in size. Yet the turbid edge was the section of morphology that had changed the most. Instead of the characteristics described above, the fuzzy, turbid edge on the plaque altered slightly into a larger sized turbid ring around the center plaque. One edge of this turbid ring thickened more than the other, with that edge flaring out as much as an extra millimeter or two past the original edge at the twenty-four hour mark. A further change was the clear ‘center’ area, which shifted towards the center of the bacteria plate, changing the appearance of KROO from a circular plaque to an oval shape. The combination of these observations: the clear, now off-center middle area and the uneven turbid ring around it gave the plaque a comet-like appearance. Each individual plaque center was oriented inwards towards the center of the plate and the trailing edges of the turbid ring stretched towards the edge of the bacterial plate. The previously larger plaques at twenty-four hours had smaller turbid rings and larger clear center areas compared to the previously smaller plaques at twenty-four hours. This was the final plaque morphology observed, complete by the end of forty-eight hours, after which the whole morphology only changed by increased overall size over time. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 14 |
| Pitt Freezer Box Grid# | 26 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
