Mycobacterium phage Kavv
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Detailed Information for Phage Kavv
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKyle Vogel and Ainelid Veras
Year Found2023
Location FoundNorth Andover, MA United States of America
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.666705 N, 71.118827 W Map
Discovery NotesAll sampling had taken place about an hour and a half before isolation at approximately 10:45 in the morning. The temperature was 70°F and with air humidity at 93% due to the current rain
storms that had previously taken place. There was a slight breeze of 1 mph winds that morning and GPS coordinates (42.6670454° N, -71.1188274° W). The sample sight I was at was a small patch of dirt that was a little grassy with a good amount of moisture and mud. The soil had been distributed with my hands with blue latex gloves on. Sampling had started after just a few inches
of digging using my hands. With my hands I initially dug down around 2 to 3 inches into the soil. At the time the soil had been very wet and muddy due to the current weather that had taken
place beforehand. After I had collected my sample, I had managed to put around a handful of soil into a clear plastic bag. I had carefully placed my right hand with the soil cupped in it, into
the bag that my left hand was holding open to support this action. After I had placed the soil into the plastic bag I had zipped the bag up and was ready to begin isolation and configure these
Naming NotesNamed after the lab partners that discovered this phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesIn these 3 plaques, obviously each one looks different from each other. In plaque 10-1 you can see there are many spots visible. There are roughly around a hundred of these spots scattered around the plaque in a grayish cloudy color, kind of like the color of a rain cloud. As you can see, the edges of this plaque has a very vibrant tan color surrounding it. In plaque 10-3 this plaque has maybe 30 of these little grayish rain cloudy spots scattered around it. It has noticeably less spots than plaque 10-1 as you can see but still has the vibrant tan ring along the edges. In plaque 10-5 you can see we had only 2 gray spots with the same tan ring and number 1 and 2 circles around them. That is because we will be using these for our next lab isolation.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 182
Pitt Freezer Box Grid# C1
Available Files
Plaque PictureDownload