Mycobacterium phage Kbarbecute
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| Detailed Information for Phage Kbarbecute | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Maggie Han |
| Year Found | 2022 |
| Location Found | Los Angeles, CA US |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.063767 N, 118.306428 W Map |
| Discovery Notes | Phage Kbarbecute was isolated from soil, collected on 3/29/22 by Maggie Han at a depth of about 2 cm from the bushes across to Hae Jang Chon AYCE KBBQ. The sample was dry and temperature at collection time was 15℃. Kbarbecute was isolated by enriched isolation in 200 mM CaCl2 cation on 20mM CaCl2 plates Wet lab characterization was performed by Team Phage-alicious (Maggie Han, Trinity Vu, Daniel Chen, Brandon Chew) MIMG103AL-S22 Instructor: Dr. Amanda Frise Teaching Assistant: Dominic Garza Undergraduate Assistants: Christopher Charton and Manar Abuwarda Laboratory Director: Dr. Krisanavane Reddi |
| Naming Notes | Our phage was named Kbarbecute because it was collected from across an all you can eat KBBQ restaurant. We also thought it was a cute pun. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Turbid during enriched isolation. During both purification assays and the titering plaque assays, it was a halo morphology. During MIDI lysate, it returned to a turbid morphology. Average 3mm; ranged between 2-5 mm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 155 |
| Pitt Freezer Box Grid# | H3 |