| Detailed Information for Phage KellEzio |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Kelly Lynch |
| Year Found | 2013 |
| Location Found | Burkesville, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.638978 N, 85.298609 W Map |
| Discovery Notes | The soil sample was taken from the base of a large tree at the lodge next to Dale Hollow Lake. The large tree was along a main sidewalk, and the sample was taken from the surface of the soil. The soil was light in color and appeared to be dry. |
| Naming Notes | 'Ezio' is the name of my favorite character from a video game. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2016 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4231 |
| Genome length (bp) | 58871 |
| Character of genome ends | Unknown |
| GC Content | 63.3% |
| Sequencing Notes | This phage has a bizarre coverage pattern. Over 95% of the reads align to one strand (the reverse strand as the final sequence is reported) and only a smattering of reads align to the other. This is highly unusual, and as of Jan 2016 has only been seen in the other Cluster AT phage, Kitkat.
Care was taken to ensure that this was not a bioinformatic artifact. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AT |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were lytic and very small. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU647626 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
