Mycobacterium phage KillerSloth
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| Detailed Information for Phage KillerSloth | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Bernaldo Castelan |
| Year Found | 2016 |
| Location Found | Austin, TX USA |
| Finding Institution | Austin Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.27 S, 97.71 E Map |
| Discovery Notes | My phage seemed to grow a t a slow rate. They also grew faster when usage of phage buffer was less. |
| Naming Notes | The name came from a sloth since it was being slow to duplicate, and killer came from killing bacteria. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques are very little and grow very slowly. They tend to grow faster when incubated. I had to do 5 spot titers. The first spot titer phage only grew in the first 3 serial dilutions. The second spot titer I had few plaques formed in my fifth dilution. The third spot titer phage grew up to my fourth dilution. My first three spot titers were created from 10ul of phage buffer instead of 3ul. For the remaining of the spot titers I used 3ul of phage buffer. My fourth spot titer I created few plaques were noticeable up to my sixth dilution, and was close to the threshold. My fifth spot titer gave me the same results as my fourth spot titer. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 34 |
| Pitt Freezer Box Grid# | B5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
