Mycobacterium phage LandoLakes
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Detailed Information for Phage LandoLakes
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNicole Lando
Year Found2015
Location FoundRye, NY United States
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature1°C
GPS CoordinatesUnavailable
Discovery NotesIsolated phage found by Dana Waxenberg in Rye, New York. The soil was collected at surface level (approximately 1-5 cm in depth) in the front yard of a suburban neighborhood. The soil was wet (sample obtained 24 hours after rainfall) and the surrounding temperature was approximately 34 degrees Fahrenheit (1 degree Celsius) and sunny.
Naming NotesThe name comes from a pun with the name of a local dairy company "LandOLakes" and also my last name.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusNot sequenced
Plaque NotesAfter 24 hours of incubation at 37 degrees Celsius, the resulting plaques were approximately 1-2 mm in diameter. These plaques were turbid with thin halos around the border of each plaque (somewhat of a bullseye look). After 48 hours of incubation, plaques exhibited similar characteristics (turbid with slightly larger halo borders), but were 3-4 mm in diameter. Titers were executed using plaques from the 72 hour incubation assay, yielding plaques of similar morphology (1-2mm in diameter after 24 hours, 3-4mm in diameter after 48 hours).
After EM imaging, it was determined that the phage is a Siphoviridae with a head to tail ratio of 1:4. The head is icosahedral in shape (although it appears spherical in the EM image). Characteristics of the tail are difficult to determine at this point, due to the limiting nature of the image's quality.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7.5E10 pfu/mL
Date of SEA Lysate Titering Mar 19, 2015
Pitt Freezer Box# 15
Pitt Freezer Box Grid# G10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload