Mycobacterium phage Lastasia
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| Detailed Information for Phage Lastasia | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jenna Currier |
| Year Found | 2019 |
| Location Found | Holland, MI United States |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.7679 N, 86.1664 W Map |
| Discovery Notes | This soil was taken just beneath fresh compost. It was very moist soil after a heavy rain. |
| Naming Notes | My phage name originated from the character Anastasia from the animated movie. I really liked her name and decided to put a spin on it. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | This phage only has a lytic cycle because it was tested for lysogen growth and yielded negative results. I took samples of bacteria that were potential lysogens from my spot dilutions and bacteria did grow. However, once these potential lysogens were placed on agar with M.smegmatis there were no plaque results, concluding that there are no lysogens produced by my phage. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | All plaques have defined edges. There is a range of sizes. There is a 0.5mm diameter turbid plaque, a 0.5mm diameter clear plaque, a 1mm diameter clear plaque, a 1.5mm diameter clear plaque, and a 2mm diameter clear plaque. The reason for the different sizes is most likely due to different start times of growth. Another interesting observation was that after refrigeration, the plaques develop a slight turbid halo. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 100 |
| Pitt Freezer Box Grid# | A5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
