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Mycobacterium phage LatifZarghona
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Detailed Information for Phage LatifZarghona
Discovery Information
Isolation HostMycobacterium smegmatis ATCC 607
Found ByShkula Babi
Year Found2019
Location FoundSanta Cruz, CA United States
Finding InstitutionUniversity of California, Santa Cruz
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS CoordinatesUnavailable
Discovery NotesBacteriophage was isolated from compost piles on the University of California, Santa Cruz (UCSC) campus. More specifically, the bacteriophage containing compost piles were located at Merrill College Garden, part of UCSC's Center for Agroecology and Sustainable Food Systems (CASFS), a known hotspot on campus for discovering bacteriophages.
Naming NotesThe phage was named LatifZarghona, after my parents as a tribute to their support in my life. If ever this phage is an effective therapeutic, LatifZarghona will play an essential role in helping support someone in life and that felt like that perfect metaphor.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Cluster Life CycleTemperate
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesSubsequent to performing serial dilutions of the phage filtrate, a total of 5 passages were carried. The phage plaques had cloudy turbidity. The initial sizes of the plaques in the serial dilution ranged from small to large. However, as the phage was purified during the passages, the plaques were a consistent medium size. The plaques had round and even edges. One issue that arose during the passages was incubation time; the phage had to be incubated for a longer period than indicated by the protocol. The titer of the final lysate for the phage that was purified was 34 x 10^(–6) plaque forming units (pfu) per mL.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload