Mycobacterium phage LatifZarghona
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| Detailed Information for Phage LatifZarghona | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Shkula Babi |
| Year Found | 2019 |
| Location Found | Santa Cruz, CA United States |
| Finding Institution | University of California, Santa Cruz |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | Bacteriophage was isolated from compost piles on the University of California, Santa Cruz (UCSC) campus. More specifically, the bacteriophage containing compost piles were located at Merrill College Garden, part of UCSC's Center for Agroecology and Sustainable Food Systems (CASFS), a known hotspot on campus for discovering bacteriophages. |
| Naming Notes | The phage was named LatifZarghona, after my parents as a tribute to their support in my life. If ever this phage is an effective therapeutic, LatifZarghona will play an essential role in helping support someone in life and that felt like that perfect metaphor. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Subsequent to performing serial dilutions of the phage filtrate, a total of 5 passages were carried. The phage plaques had cloudy turbidity. The initial sizes of the plaques in the serial dilution ranged from small to large. However, as the phage was purified during the passages, the plaques were a consistent medium size. The plaques had round and even edges. One issue that arose during the passages was incubation time; the phage had to be incubated for a longer period than indicated by the protocol. The titer of the final lysate for the phage that was purified was 34 x 10^(–6) plaque forming units (pfu) per mL. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
