Mycobacterium phage LeCap
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Detailed Information for Phage LeCap
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesLeCure
Found ByAria Ashoury; Gavin Channell
Year Found2023
Location FoundBoulder, CO United States of America
Finding InstitutionUniversity of Colorado Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.009001 N, 105.269074 W Map
Discovery NotesThe soil sample was collected along a walking path in the middle of a college campus, next to a bush. The sample was collected from (40.0090008, -105.2690736) at 7°C (45°F). Approximately 3 mL of the collected sample was incubated in 4mL LB media (10g tryptone, 5g yeast extract, 10g NaCl) and 1 mL Mycobacterium smegmatis (mc^2 155) and the result was incubated (shaking) for 48 hours at 37°C.
Naming NotesThe phage name was inspired by LeBron James.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaque morphology was identified as all temperate phages that were 4mm +/- 1mm in diameter with cloudy “halos” present in some form around the edges. The final titer of the high titer lysate was calculated as 7.7E9 pfu/mL.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 178
Pitt Freezer Box Grid# D4
Available Files
Plaque PictureDownload
EM PictureDownload