Mycobacterium phage LeCap
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| Detailed Information for Phage LeCap | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | LeCure |
| Found By | Aria Ashoury; Gavin Channell |
| Year Found | 2023 |
| Location Found | Boulder, CO United States of America |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.009001 N, 105.269074 W Map |
| Discovery Notes | The soil sample was collected along a walking path in the middle of a college campus, next to a bush. The sample was collected from (40.0090008, -105.2690736) at 7°C (45°F). Approximately 3 mL of the collected sample was incubated in 4mL LB media (10g tryptone, 5g yeast extract, 10g NaCl) and 1 mL Mycobacterium smegmatis (mc^2 155) and the result was incubated (shaking) for 48 hours at 37°C. |
| Naming Notes | The phage name was inspired by LeBron James. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaque morphology was identified as all temperate phages that were 4mm +/- 1mm in diameter with cloudy “halos” present in some form around the edges. The final titer of the high titer lysate was calculated as 7.7E9 pfu/mL. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 178 |
| Pitt Freezer Box Grid# | D4 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |