Mycobacterium phage LeCap
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Detailed Information for Phage LeCap | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | LeCure |
Found By | Aria Ashoury; Gavin Channell |
Year Found | 2023 |
Location Found | Boulder, CO United States of America |
Finding Institution | University of Colorado Boulder |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 40.009001 N, 105.269074 W Map |
Discovery Notes | The soil sample was collected along a walking path in the middle of a college campus, next to a bush. The sample was collected from (40.0090008, -105.2690736) at 7°C (45°F). Approximately 3 mL of the collected sample was incubated in 4mL LB media (10g tryptone, 5g yeast extract, 10g NaCl) and 1 mL Mycobacterium smegmatis (mc^2 155) and the result was incubated (shaking) for 48 hours at 37°C. |
Naming Notes | The phage name was inspired by LeBron James. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The plaque morphology was identified as all temperate phages that were 4mm +/- 1mm in diameter with cloudy “halos” present in some form around the edges. The final titer of the high titer lysate was calculated as 7.7E9 pfu/mL. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
Pitt Freezer Box# | 178 |
Pitt Freezer Box Grid# | D4 |
Available Files | |
Plaque Picture | Download |
EM Picture | Download |