Gordonia phage LilSebastien
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| Detailed Information for Phage LilSebastien | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Zoe Delozier |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | Unavailable |
| Discovery Notes | This phage was collected from a planter in a courtyard at the University of Pittsburgh. On the day of the soil collection, 1/12/16, there was 3-4 inches of snow on the ground and the temperature haven't been above 30 degrees Fahrenheit for days at a time. Due to these weather conditions, it was challenging to collect the soil because I had to push the snow and the top mulch layer out of the way to get to the dirt. Once I got to the dirt, I realized it was frozen solids had to use a metal fork and spoon like a rake and shovel to collect my sample. This phage was found in a planter that had scrubs and trees that were healthy. |
| Naming Notes | My phage's name is inspired by a "character" from Parks and Rec, a popular TV show starring Amy Poehler. LilSebastian is a pony that the entire town is obsessed with, but outsiders do not understand what the big deal is. Like the character LilSebastian, my phage is small and a big deal to me and the SEA-PHAGES community, even though outsiders may not understand why. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Forms a lysogen, own lysate did not infect lysogen |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | LilSebastian's plaques are slightly ovular with cloudy edges and the center is clear which gives it the appearance of a grey dot compared to the cloudy border around it. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2/8/16 |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | E2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
